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sheep polyclonal antibodies against human tigit  (R&D Systems)


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    R&D Systems sheep polyclonal antibodies against human tigit
    Sheep Polyclonal Antibodies Against Human Tigit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibodies+against+fap/Human+Fibroblast+Activation+Protein+alpha%2FFAP+Antibody/pm40298304-62-9-15
    Average 95 stars, based on 98 article reviews
    sheep polyclonal antibodies against human tigit - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Suspension:

    Article Title: The dual role of asporin in breast cancer progression
    Article Snippet: To confirm homogeneity of isolated fibroblasts (denoted as BCAF3, BCAF4 and BCAF5), we performed FACS analysis of three selected surface fibroblast markers using BD FACSVerse flow cytometer. .. Cell suspension obtained by trypsinisation was stained with primary antibodies against FAP (human fibroblast activation protein alpha, MAB3715, R&D Systems), FSP (fibroblast surface protein, F4771, Sigma-Aldrich), followed by staining with secondary antibody Alexa Fluor 488 conjugated donkey anti-mouse IgG (H+L) (A21202, ThermoFisher Scientific); and primary antibody anti-fibroblast FITC (130-100-134, Miltenyi Biotec). .. Dead cells were excluded from the analysis using LIVE/DEAD Fixable Far Red Dead Cell Stain Kit (L10120, ThermoFisher Scientific).

    Article Title: The dual role of asporin in breast cancer progression.
    Article Snippet: To confirm homogeneity of isolated fibroblasts (denoted as BCAF3, BCAF4 and BCAF5), we performed FACS analysis of three selected surface fibroblast markers using BD FACSVerse flow cytometer. .. Cell suspension obtained by trypsinisation was stained with primary antibodies against FAP (human fibroblast activation protein alpha, MAB3715, R&D Systems), FSP (fibroblast surface protein, F4771, SigmaAldrich), followed by staining with secondary antibody Alexa Fluor 488 conjugated donkey anti-mouse IgG (H+L) (A21202, ThermoFisher Scientific); and primary antibody anti-fibroblast FITC (130-100-134, Miltenyi Biotec). .. Dead cells were excluded from the analysis using LIVE/ DEAD Fixable Far Red Dead Cell Stain Kit (L10120, ThermoFisher Scientific).

    Staining:

    Article Title: The dual role of asporin in breast cancer progression
    Article Snippet: To confirm homogeneity of isolated fibroblasts (denoted as BCAF3, BCAF4 and BCAF5), we performed FACS analysis of three selected surface fibroblast markers using BD FACSVerse flow cytometer. .. Cell suspension obtained by trypsinisation was stained with primary antibodies against FAP (human fibroblast activation protein alpha, MAB3715, R&D Systems), FSP (fibroblast surface protein, F4771, Sigma-Aldrich), followed by staining with secondary antibody Alexa Fluor 488 conjugated donkey anti-mouse IgG (H+L) (A21202, ThermoFisher Scientific); and primary antibody anti-fibroblast FITC (130-100-134, Miltenyi Biotec). .. Dead cells were excluded from the analysis using LIVE/DEAD Fixable Far Red Dead Cell Stain Kit (L10120, ThermoFisher Scientific).

    Article Title: Molecular Imaging of Fibroblast Activity After Myocardial Infarction Using a 68 Ga-Labeled Fibroblast Activation Protein Inhibitor, FAPI-04
    Article Snippet: .. Tissue sections were stained with antibodies against FAP (R&D Systems) as a marker of activated fibroblasts, prolyl-4-hydroxylase β (Origene) as a marker of activated and collagen-synthesizing fibroblasts, α-smooth muscle actin (Merck) as a marker of differentiated mature myofibroblasts, and vimentin (Merck) as a general fibroblast marker, as described previously ( 8 ). .. Tissue sections were stained with antibodies against FAP (R&D Systems) as a marker of activated fibroblasts, prolyl-4-hydroxylase β (Origene) as a marker of activated and collagen-synthesizing fibroblasts, α-smooth muscle actin (Merck) as a marker of differentiated mature myofibroblasts, and vimentin (Merck) as a general fibroblast marker, as described previously ( 8 ).

    Article Title: The dual role of asporin in breast cancer progression.
    Article Snippet: To confirm homogeneity of isolated fibroblasts (denoted as BCAF3, BCAF4 and BCAF5), we performed FACS analysis of three selected surface fibroblast markers using BD FACSVerse flow cytometer. .. Cell suspension obtained by trypsinisation was stained with primary antibodies against FAP (human fibroblast activation protein alpha, MAB3715, R&D Systems), FSP (fibroblast surface protein, F4771, SigmaAldrich), followed by staining with secondary antibody Alexa Fluor 488 conjugated donkey anti-mouse IgG (H+L) (A21202, ThermoFisher Scientific); and primary antibody anti-fibroblast FITC (130-100-134, Miltenyi Biotec). .. Dead cells were excluded from the analysis using LIVE/ DEAD Fixable Far Red Dead Cell Stain Kit (L10120, ThermoFisher Scientific).

    Activation Assay:

    Article Title: The dual role of asporin in breast cancer progression
    Article Snippet: To confirm homogeneity of isolated fibroblasts (denoted as BCAF3, BCAF4 and BCAF5), we performed FACS analysis of three selected surface fibroblast markers using BD FACSVerse flow cytometer. .. Cell suspension obtained by trypsinisation was stained with primary antibodies against FAP (human fibroblast activation protein alpha, MAB3715, R&D Systems), FSP (fibroblast surface protein, F4771, Sigma-Aldrich), followed by staining with secondary antibody Alexa Fluor 488 conjugated donkey anti-mouse IgG (H+L) (A21202, ThermoFisher Scientific); and primary antibody anti-fibroblast FITC (130-100-134, Miltenyi Biotec). .. Dead cells were excluded from the analysis using LIVE/DEAD Fixable Far Red Dead Cell Stain Kit (L10120, ThermoFisher Scientific).

    Article Title: Cancer-associated fibroblasts derived fibronectin extra domain A promotes sorafenib resistance in hepatocellular carcinoma cells by activating SHMT1
    Article Snippet: .. The cells on slides were fixed with 4% paraformaldehyde for 10 min and permeabilized in phosphate buffer saline for 20 min. Then, cells were blocked with goat serum at room temperature for 60 min and incubated with primary antibodies against FAP (fibroblast activation protein; R&D system, #FAB3715A, RRID: AB_2884010) (1:200) and α-SMA (alpha-smooth muscle actin; R&D system, #MAB1420, RRID: AB_262054) (1:200) for 2 h. Then, the cells on slides were reheated and incubated with the corresponding secondary antibody at 37 °C in the dark for 2 h. Nuclei were counter-stained with DAPI. .. An annexin V-FITC/PI assay (Bestbio, BB-4101) was used to measure apoptotic cells by flow cytometry according to the manufacturer's instructions.

    Article Title: The dual role of asporin in breast cancer progression.
    Article Snippet: To confirm homogeneity of isolated fibroblasts (denoted as BCAF3, BCAF4 and BCAF5), we performed FACS analysis of three selected surface fibroblast markers using BD FACSVerse flow cytometer. .. Cell suspension obtained by trypsinisation was stained with primary antibodies against FAP (human fibroblast activation protein alpha, MAB3715, R&D Systems), FSP (fibroblast surface protein, F4771, SigmaAldrich), followed by staining with secondary antibody Alexa Fluor 488 conjugated donkey anti-mouse IgG (H+L) (A21202, ThermoFisher Scientific); and primary antibody anti-fibroblast FITC (130-100-134, Miltenyi Biotec). .. Dead cells were excluded from the analysis using LIVE/ DEAD Fixable Far Red Dead Cell Stain Kit (L10120, ThermoFisher Scientific).

    Saline:

    Article Title: Cancer-associated fibroblasts derived fibronectin extra domain A promotes sorafenib resistance in hepatocellular carcinoma cells by activating SHMT1
    Article Snippet: .. The cells on slides were fixed with 4% paraformaldehyde for 10 min and permeabilized in phosphate buffer saline for 20 min. Then, cells were blocked with goat serum at room temperature for 60 min and incubated with primary antibodies against FAP (fibroblast activation protein; R&D system, #FAB3715A, RRID: AB_2884010) (1:200) and α-SMA (alpha-smooth muscle actin; R&D system, #MAB1420, RRID: AB_262054) (1:200) for 2 h. Then, the cells on slides were reheated and incubated with the corresponding secondary antibody at 37 °C in the dark for 2 h. Nuclei were counter-stained with DAPI. .. An annexin V-FITC/PI assay (Bestbio, BB-4101) was used to measure apoptotic cells by flow cytometry according to the manufacturer's instructions.

    Incubation:

    Article Title: Cancer-associated fibroblasts derived fibronectin extra domain A promotes sorafenib resistance in hepatocellular carcinoma cells by activating SHMT1
    Article Snippet: .. The cells on slides were fixed with 4% paraformaldehyde for 10 min and permeabilized in phosphate buffer saline for 20 min. Then, cells were blocked with goat serum at room temperature for 60 min and incubated with primary antibodies against FAP (fibroblast activation protein; R&D system, #FAB3715A, RRID: AB_2884010) (1:200) and α-SMA (alpha-smooth muscle actin; R&D system, #MAB1420, RRID: AB_262054) (1:200) for 2 h. Then, the cells on slides were reheated and incubated with the corresponding secondary antibody at 37 °C in the dark for 2 h. Nuclei were counter-stained with DAPI. .. An annexin V-FITC/PI assay (Bestbio, BB-4101) was used to measure apoptotic cells by flow cytometry according to the manufacturer's instructions.

    Marker:

    Article Title: Molecular Imaging of Fibroblast Activity After Myocardial Infarction Using a 68 Ga-Labeled Fibroblast Activation Protein Inhibitor, FAPI-04
    Article Snippet: .. Tissue sections were stained with antibodies against FAP (R&D Systems) as a marker of activated fibroblasts, prolyl-4-hydroxylase β (Origene) as a marker of activated and collagen-synthesizing fibroblasts, α-smooth muscle actin (Merck) as a marker of differentiated mature myofibroblasts, and vimentin (Merck) as a general fibroblast marker, as described previously ( 8 ). .. Tissue sections were stained with antibodies against FAP (R&D Systems) as a marker of activated fibroblasts, prolyl-4-hydroxylase β (Origene) as a marker of activated and collagen-synthesizing fibroblasts, α-smooth muscle actin (Merck) as a marker of differentiated mature myofibroblasts, and vimentin (Merck) as a general fibroblast marker, as described previously ( 8 ).



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    (a–c) Flow-cytometry workflow and representative plots for (a) NF alone, (b) NF co-cultured with RT112, and (c) NF co-cultured with T24 for 48 h. Gates show total cells (FSC-A vs SSC-A), singlets (FSC-H vs FSC-A), and the HBSF population identified by Alexa Fluor 488 negative (pre-labelled cancer with CellTracker Green). The frequency of gated HBSF within each condition is indicated. Right-hand histograms display activation-marker staining in gated HBSF <t>(αSMA/FAP;</t> Alexa Fluor 647 channel). (d) Quantification of mean fluorescence intensity (MFI) for αSMA and FAP in HBSF monoculture versus direct co-culture with RT112 or T24 (48 h). Both co-cultures significantly increased <t>fibroblast</t> activation marker expression, with the strongest induction observed in T24– HBSF. Bars show mean ± SD from n = 3.
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    sheep polyclonal antibodies against human tigit  (R&D Systems)
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    antibody against mouse fap r d systems  (R&D Systems)
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    Figure 8. Immunohistochemical evaluation of excised tumor tissues from TUBO tumor-bearing Balb/C mice. Brown signals correspond to <t>anti-FAP</t> staining; nuclei were counterstained with hematoxylin. Immunohistochemical staining of excised tumor tissue in the absence of primary antibody anti-FAP above and immunohistochemical staining of excised tumor tissue (anti-FAP primary antibody) below.
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    Image Search Results


    (a–c) Flow-cytometry workflow and representative plots for (a) NF alone, (b) NF co-cultured with RT112, and (c) NF co-cultured with T24 for 48 h. Gates show total cells (FSC-A vs SSC-A), singlets (FSC-H vs FSC-A), and the HBSF population identified by Alexa Fluor 488 negative (pre-labelled cancer with CellTracker Green). The frequency of gated HBSF within each condition is indicated. Right-hand histograms display activation-marker staining in gated HBSF (αSMA/FAP; Alexa Fluor 647 channel). (d) Quantification of mean fluorescence intensity (MFI) for αSMA and FAP in HBSF monoculture versus direct co-culture with RT112 or T24 (48 h). Both co-cultures significantly increased fibroblast activation marker expression, with the strongest induction observed in T24– HBSF. Bars show mean ± SD from n = 3.

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    Article Title: Bidirectional Crosstalk Between Bladder Cancer Cells and Normal Fibroblasts Drives Phenotypic Reprogramming and Modulates Chemosensitivity

    doi: 10.1101/2025.09.09.675061

    Figure Lengend Snippet: (a–c) Flow-cytometry workflow and representative plots for (a) NF alone, (b) NF co-cultured with RT112, and (c) NF co-cultured with T24 for 48 h. Gates show total cells (FSC-A vs SSC-A), singlets (FSC-H vs FSC-A), and the HBSF population identified by Alexa Fluor 488 negative (pre-labelled cancer with CellTracker Green). The frequency of gated HBSF within each condition is indicated. Right-hand histograms display activation-marker staining in gated HBSF (αSMA/FAP; Alexa Fluor 647 channel). (d) Quantification of mean fluorescence intensity (MFI) for αSMA and FAP in HBSF monoculture versus direct co-culture with RT112 or T24 (48 h). Both co-cultures significantly increased fibroblast activation marker expression, with the strongest induction observed in T24– HBSF. Bars show mean ± SD from n = 3.

    Article Snippet: For NF, primary antibodies against fibroblast activation protein (FAP) and α-smooth muscle actin (αSMA) (Cell Signaling Technology) were applied at 1:100 overnight at 4°C.

    Techniques: Flow Cytometry, Cell Culture, Activation Assay, Marker, Staining, Fluorescence, Co-Culture Assay, Expressing

    Figure 8. Immunohistochemical evaluation of excised tumor tissues from TUBO tumor-bearing Balb/C mice. Brown signals correspond to anti-FAP staining; nuclei were counterstained with hematoxylin. Immunohistochemical staining of excised tumor tissue in the absence of primary antibody anti-FAP above and immunohistochemical staining of excised tumor tissue (anti-FAP primary antibody) below.

    Journal: Molecular pharmaceutics

    Article Title: Novel FAP-Targeted Heptamethine Cyanines for NIRF Imaging Applications.

    doi: 10.1021/acs.molpharmaceut.4c01232

    Figure Lengend Snippet: Figure 8. Immunohistochemical evaluation of excised tumor tissues from TUBO tumor-bearing Balb/C mice. Brown signals correspond to anti-FAP staining; nuclei were counterstained with hematoxylin. Immunohistochemical staining of excised tumor tissue in the absence of primary antibody anti-FAP above and immunohistochemical staining of excised tumor tissue (anti-FAP primary antibody) below.

    Article Snippet: FAP expression was measured by Western Blot using antibodies against FAP (Santa Cruz, sc-100528) and β actin as a loading control (Santa Cruz, sc-4778).

    Techniques: Immunohistochemical staining, Staining